Hi,
> I do not have IIS installed.
You must have some IIS installed, otherwise port 80 would be free.
Since you have the -Indexes option for your VirtualHost, do you have an index.php file in the D:/wamp64/www/iseppL/ folder?
In the address bar of Chrome, it says 'http://iseppL:8081/' ?
Because if 'http://localhost:8081/' works, there is no reason why 'http://iseppL:8081/' should not work.
I just did a full-scale test by changing the listening port 80 to 8081 via :
Right-click -> Tools -> Use a port other than 80 and all my VirtualHost work, even with Chrome.
Check with another browser like Firefox.
Note: You may need to clear the browser cache with Ctrl-F5
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